Journal: International Journal of Oncology

Article Title: Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma

doi: 10.3892/ijo.2016.3601

Figure Lengend Snippet: Characteristics of patients with osteosarcoma and non-cancer participants.

Article Snippet: Osteosarcoma cell line (MMNG-HOS) catalog no. CLS 300289 passage 24 and U2OS catalog no. CLS 300364 passage 24 were used as representative osteosarcoma cells.

Techniques: Biomarker Discovery

Journal: International Journal of Oncology

Article Title: Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma

doi: 10.3892/ijo.2016.3601

Figure Lengend Snippet: Representative 2D-PAGE of osteosarcoma and osteoblast. (A) Red arrows show spots significantly upregulated in osteosarcomas; green arrows show spots significantly upregulated in osteoblasts. (B) Heat map shows fold changes of % volume of protein spots between osteosarcoma and osteoblast in each matched set.

Article Snippet: Osteosarcoma cell line (MMNG-HOS) catalog no. CLS 300289 passage 24 and U2OS catalog no. CLS 300364 passage 24 were used as representative osteosarcoma cells.

Techniques:

Journal: International Journal of Oncology

Article Title: Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma

doi: 10.3892/ijo.2016.3601

Figure Lengend Snippet: Summary of significant altered proteins in primary osteosarcoma cells identified by LC-MS/MS.

Article Snippet: Osteosarcoma cell line (MMNG-HOS) catalog no. CLS 300289 passage 24 and U2OS catalog no. CLS 300364 passage 24 were used as representative osteosarcoma cells.

Techniques:

Journal: International Journal of Oncology

Article Title: Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma

doi: 10.3892/ijo.2016.3601

Figure Lengend Snippet: (A) KSRP expression levels in experimental samples (1, pooled sample of osteosarcoma; 2, pool sample of osteoblasts; 3–15 (odd number), individual osteosarcoma cases; 2–16 (even number), individual osteoblast cases; 17, MNNG-HOS; 18, U2OS; 19, HeLa cells as the positive control). (B) KSRP expression in 12 representative biopsy samples of osteosarcoma cases in the separate group.

Article Snippet: Osteosarcoma cell line (MMNG-HOS) catalog no. CLS 300289 passage 24 and U2OS catalog no. CLS 300364 passage 24 were used as representative osteosarcoma cells.

Techniques: Expressing, Positive Control

Journal: International Journal of Oncology

Article Title: Overexpression of KH-type splicing regulatory protein regulates proliferation, migration, and implantation ability of osteosarcoma

doi: 10.3892/ijo.2016.3601

Figure Lengend Snippet: Significantly decreased migratory behavior of osteosarcoma cell lines after KSRP knock-down by siRNA-1 (siR-1) and si-RNA2 (siR-2) compared with non-sense (NS) siRNA as a control (A) at 48 h and (B) at 72 h. (C) Cell growth curve showing significantly decreased proliferative ability of osteosarcoma cell lines after KSRP knock-down.

Article Snippet: Osteosarcoma cell line (MMNG-HOS) catalog no. CLS 300289 passage 24 and U2OS catalog no. CLS 300364 passage 24 were used as representative osteosarcoma cells.

Techniques: Knockdown, Control

Journal: Marine Drugs

Article Title: Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

doi: 10.3390/md16120504

Figure Lengend Snippet: IC 50 of EPP after 24 and 48 h treatment on SaOS-2 and MNNG. IC 50 was calculated using Graphpad. Cell viability was expressed as percentage in respect to control and EPP concentrations (3.1, 6.25, 12.5, 25, 50, 100 and 200 μg/mL) were reported in a logarithmic scale.

Article Snippet: Human OS SaOS-2 (ATCC-HTB-85) and MNNG (ATCC-CRL-1547) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured as described [ , , ] in DMEM containing 10% v/v FBS, 100 mg/mL penicillin and 100 mg/mL streptomycin.

Techniques: Control

Journal: Marine Drugs

Article Title: Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

doi: 10.3390/md16120504

Figure Lengend Snippet: SaOS-2 and MNNG cell proliferation after 24 h treatment with EPP at IC 50 /2, IC 50 and 2*IC 50 in starved conditions determined by the Scepter 2.0 cell counter. Data are expressed as percentage in respect to control and presented as mean ± SD, **** p < 0.0001.

Article Snippet: Human OS SaOS-2 (ATCC-HTB-85) and MNNG (ATCC-CRL-1547) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured as described [ , , ] in DMEM containing 10% v/v FBS, 100 mg/mL penicillin and 100 mg/mL streptomycin.

Techniques: Control

Journal: Marine Drugs

Article Title: Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

doi: 10.3390/md16120504

Figure Lengend Snippet: ( A ) Bright-field images of SaOS-2 and MNNG OS cells line after 24 h treatment with EPP at IC 50 /2, IC 50 and 2*IC 50 or DMSO 0.3% as negative control. Cells are shown at ×10 magnification. ( B ) Nuclear morphological changes and DNA damage assessment in SaOS-2 and MNNG cells OS cells line after 24 h treatment with EPP at IC 50 /2, IC 50 and 2*IC 50 using DMSO 0.3% as negative control. Arrows indicate nuclear fragmentation, which can be considered a biochemical hallmark of apoptosis. Cells are shown at ×63 magnification.

Article Snippet: Human OS SaOS-2 (ATCC-HTB-85) and MNNG (ATCC-CRL-1547) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured as described [ , , ] in DMEM containing 10% v/v FBS, 100 mg/mL penicillin and 100 mg/mL streptomycin.

Techniques: Negative Control

Journal: Marine Drugs

Article Title: Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

doi: 10.3390/md16120504

Figure Lengend Snippet: Diameter distributions of SaOS-2 and MNNG cells after 24 h treatment with EPP at IC 50 /2, IC 50 and 2*IC 50 as measured by Scepter 2.0 cell counter. The cells were classified into small (diameter: 6–9 µm) or large (diameter 9–24 µm) fractions depending on the gating range.

Article Snippet: Human OS SaOS-2 (ATCC-HTB-85) and MNNG (ATCC-CRL-1547) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured as described [ , , ] in DMEM containing 10% v/v FBS, 100 mg/mL penicillin and 100 mg/mL streptomycin.

Techniques:

Journal: Marine Drugs

Article Title: Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

doi: 10.3390/md16120504

Figure Lengend Snippet: ( A ) Apoptosis assessment using Annexin V-FITC/PI staining and fluorescent microscopy of SaOS-2 and MNNG OS cells line treated with EPP/Acetone (IC 50 /2, IC 50 , 2*IC 50 ) for 6 h. Viable cells did not show any kind of coloration (Anv−/PI−). Cells stained in green (AnV+/PI−) were considered as early apoptotic cells, while cells stained both in green and red (Anv+/PI−) were considered as late apoptotic cells. Cells are shown at ×40 magnification. Arrows indicate co-localization. ( B ) Histograms show the percentage of non-apoptotic(AnV−/PI−), early apoptotic (AnV+/PI−) and late apoptotic (AnV+/PI+) cells in respect to control. The quantitative assessment of apoptosis was obtained observing merged images. Results were obtained from three different experiments in triplicate, **** p < 0.0001.

Article Snippet: Human OS SaOS-2 (ATCC-HTB-85) and MNNG (ATCC-CRL-1547) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured as described [ , , ] in DMEM containing 10% v/v FBS, 100 mg/mL penicillin and 100 mg/mL streptomycin.

Techniques: Staining, Microscopy, Control

Journal: Marine Drugs

Article Title: Pro-Apoptotic Activity of French Polynesian Padina pavonica Extract on Human Osteosarcoma Cells

doi: 10.3390/md16120504

Figure Lengend Snippet: EPP treated SaOS-2 and MNNG OS cells line western blotting images of procaspase-3, cleaved caspase-3 and GAPDH. Data are expressed as fold-change in respect to control and presented as mean ± SD. Results were obtained from three different experiments in triplicate, **** p < 0.0001.

Article Snippet: Human OS SaOS-2 (ATCC-HTB-85) and MNNG (ATCC-CRL-1547) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured as described [ , , ] in DMEM containing 10% v/v FBS, 100 mg/mL penicillin and 100 mg/mL streptomycin.

Techniques: Western Blot, Control